Optogenetic induction of neurodegenerative disease pathology

摘要

The present disclosure relates to compounds, compositions, and methods for inducing neurodegenerative disease pathology. In one aspect, disclosed herein is a nucleotide sequence that encodes a chimeric polypeptide that includes a first nucleotide sequence that encodes a light-induced oligomerization domain and a second nucleotide sequence that encodes a neurodegenerative disease target protein. . A method of inducing a neurodegenerative disease pathology in a cell comprising a first nucleotide sequence encoding a light-induced oligomerization domain and a second nucleotide sequence encoding a low complexity domain from a neurodegenerative disease target protein. An expression vector encoding a chimeric polypeptide, wherein the first nucleotide sequence is operably linked to a promoter, the step of introducing the expression vector into the cell; the step of expressing the chimeric polypeptide; and the blue light Disclosed herein are methods that include the step of inducing oligomerization of the chimeric polypeptide upon stimulation used.