Highly specific circular proximity ligation assay

摘要

Provided herein is a circular proximity ligation assay in which a proximity probe is used as a bridge, connecting two free oligonucleotides via a double ligation event, resulting in the formation of a circle. The circle is then quantified, for example by qPCR. It is believed that the addition of extra oligonucleotides increases specificity by reducing the probability of random background ligation events. In addition, circular formation can have selective advantages, as simple exonuclease treatment can remove non-circularized DNA, which streamlined the workflow by eliminating pre-amplification prior to qPCR. [Selection diagram] None