Three-dimensional in vitro alveoli model, method of making the model, and use thereof to measure and / or predict sensitizing effects of inhalable products

摘要

The present invention relates essentially to a three-dimensional in vitro alveolar model comprising four cell types: (lung-producing) alveolar type II epithelial cells capable of secreting surfactant; capillaries providing a permeable barrier Endothelial cells that form the inner wall of cells; dendritic-like cells such as undifferentiated THP-1 that link innate and adaptive immunity; and macrophage-like cells that can participate in defense mechanisms by ingesting foreign substances through phagocytosis. The present invention also relates to a method for producing said model, further for assessing the irritation or toxicity of an inhalable product, such as particles or molecules at the alveolar barrier of the lung, as well as particles or molecules at the alveolar barrier of the lung. And its use for measuring and / or predicting the sensitizing effect of an inhalable product. [Selection diagram] Fig. 1