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Position detection method for sample container, imaging method using the same, and position detection device for sample container

机译:样本容器的位置检测方法,使用其的成像方法以及样本容器的位置检测装置

摘要

There is provided a method of detecting positional displacement of a sample container placed on an imaging device, at high speed and with high accuracy without increasing the amount of data required for a process. Two wells are selected (step S10) and upper, lower, left, and right logical edge coordinates of the two wells are calculated (step S20). An image of a portion near each set of logical edge coordinates is captured (step S30), and a rectangular separability filter is applied to each captured image and for each captured image, coordinates of a center position of the rectangular separability filter obtained when a peak value of a separability is obtained are detected as temporary edge coordinates (step S40). Thereafter, actual center coordinates are calculated for each well selected in step S10, based on upper, lower, left, and right temporary edge coordinates (step S60) . Finally, the amount of positional displacement of a well plate from an ideal placement state is calculated based on the center coordinates of the two wells selected in step S10 (step S70).
机译:提供了一种在不增加处理所需的数据量的情况下高速且高精度地检测放置在成像装置上的样品容器的位置位移的方法。选择两个井(步骤S10),并计算两个井的上,下,左和右逻辑边缘坐标(步骤S20)。捕获每组逻辑边缘坐标附近的部分的图像(步骤S30),并且将矩形可分离性滤波器应用于每个捕获的图像,并且对于每个捕获的图像,当峰值达到峰值时获得矩形可分离性滤波器的中心位置的坐标。将获得的可分离性的值检测为临时边缘坐标(步骤S40)。此后,基于上,下,左和右临时边缘坐标,为在步骤S10中选择的每个井计算实际中心坐标(步骤S60)。最后,基于在步骤S10中选择的两个孔的中心坐标计算孔板相对于理想放置状态的位置偏移量(步骤S70)。

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