Methods for Producing and Using Sinoatrial Node Pacemaker Cardiomyocytes and Ventricular Cardiomyocytes

摘要

PROBLEM TO BE SOLVED: To provide a method for producing a sinoatrial node-like pacemaker cardiomyocyte (SANLCM) and a ventricular-like cardiomyocyte (VLCM) from human pluripotent stem cells (hPSC). A method for producing a cardiomyocyte population, wherein a. Cardiovascular mesoderm cells are incubated for a period of time in a cardiac induction medium containing a WNT inhibitor, VEGF, or both to produce cardiovascular progenitor cells expressing NKX2-5; b. A method comprising the step of incubating the cardiovascular progenitor cells in a basal medium containing VEGF for a period of time to generate a cardiomyocyte population rich in NKX2-5 ^pos cTnT ^pos cells. [Selection diagram] None