Method of increasing pyramidal photoreceptors or rod photoreceptors by dorsal or ventral signaling

摘要

The present invention provides a retinal tissue rich in pyramidal photoreceptor cells and / or pyramidal photoreceptors, a retinal tissue rich in rod photoreceptors and / or rod photoreceptors, and a method for producing them. Make it an issue. The retinal tissue from the early stage of development to the stage where the appearance rate of pyramidal photoreceptor progenitors is maximized is cultured in a medium containing i) a dorsal signaling substance having a concentration sufficient to suppress the expression of ventral markers. A method of increasing the proportion of pyramidal photoreceptors and pyramidal photoreceptors in the photoreceptors and photoreceptors contained in the retinal tissue, including the step, or ii) a concentration that promotes the expression of ventral markers. A method of increasing the proportion of rod photoreceptors and rod photoreceptors in the photoreceptor cells and photoreceptors contained in the retinal tissue, comprising the step of culturing in the presence of a ventral signaling substance.