Collective quantification of target proteins using next-generation sequencing and its applications

摘要

The present invention discloses a method for collectively quantifying a target protein using next-generation sequencing. The method of the present invention comprises the steps of: (a) subjecting a sample to be analyzed to a target protein population present in the sample and treating the aptamer library to form a complex between each target protein and each aptamer that specifically binds thereto; Thereby forming a complex population of the target protein and the aptamer; (b) separating the complex population from unbound aptamers; and (c) changing the sequence of each aptamer in the complex population. Quantifying each target protein of the complex population by analyzing with a generation sequence analysis technique and quantifying each aptamer of the complex population. The method of the present invention can be very usefully used for collectively quantifying proteins in an analysis sample. [Selection diagram] None