Isobaric aldehyde-reactive tags for and analysis of glycans using same

摘要

Highly specific and novel methods for analyzing glycans and proteoglycans are provided. The present invention provides glycan-reactive isobaric Aldehyde-Reactive Tags (iARTs) and the quantification of iARTs-labeled glycans by tandem mass spectrometry. The iARTs have an amine as an active group to react with aldehyde at the reducing end of glycans through reductive amination and demonstrated complete labeling. Due to the isobaric nature of the iARTs, differentially labeled glycans do not differ in mass, and quantitative information is provided by the isotope-encoded reporter ions generated from MS/MS or MS³ spectra. Quantitative information is thus derived from signal of the reporter ions on same precursor. Uses of the information generated by the inventive methods for diagnosis and treatment are also disclosed.