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Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
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机译:用于合成测序的相保护试剂流排序
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摘要
A method for nucleic acid sequencing includes: disposing a plurality of template polynucleotide strands, sequencing primers, and polymerases in a plurality of defined spaces of a sensor array; exposing template polynucleotide strands to a series of flows of nucleotide species, the series comprising a sequence of random flows; and obtaining, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.
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