首页>
外国专利>
Isolation, expansion and characterization of wharton's jelly mesenchymal stem cells
Isolation, expansion and characterization of wharton's jelly mesenchymal stem cells
展开▼
机译:沃顿氏胶质间充质干细胞的分离,扩增和鉴定
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to a method of isolating mesenchymal stem cells originated from Wharton's jelly (WJ-MSC) from single donor. The method for isolating and proliferating viable cells from Wharton's jelly wherein the method includes the steps of (a) obtaining umbilical cord tissue comprising of viable cells from Wharton's jelly, (b) isolating the viable cells in a sample collection media, (c) preparing a primary stock culture using the viable cells from step (b), (d) proliferating the primary stock culture from step (c) to obtain sub-cultured stocks, (e) harvesting the viable cells from the sub-cultured stocks from step (d) and (f) cryo-preserving the viable cells from step (e) under cryogenic conditions.
展开▼