首页> 外国专利> Method of culturing Borrelia spirochetes and of diagnosing Borrelia infections, and a diagnostic kit for use in the methods

Method of culturing Borrelia spirochetes and of diagnosing Borrelia infections, and a diagnostic kit for use in the methods

机译：培养鲍氏螺旋体的方法和诊断鲍氏螺旋体感染的方法以及用于该方法的诊断试剂盒

页面导航

摘要
著录项
相似文献

摘要

Herein is provided a method that provides a patient, suspected of having Lyme disease, a kit that does not require specialized equipment and allows for easy self-collection of specimens. Methods provided herein, allow for the efficient culturing of Borrelia spirochetes, such as B. burgdorferi spirochetes after being exposed to an environment that is typically not suitable for Borrelia growth.

机译：本文提供了一种方法，该方法为怀疑患有莱姆病的患者提供了一种试剂盒，该试剂盒不需要专门的设备，并且易于标本的自我收集。本文提供的方法允许有效培养 Borrelia 螺旋形体，例如 B。在暴露于通常不适合勃氏疏螺旋体生长的环境中后，勃氏飞蛾会飞出。 展开▼

著录项

公开/公告号US10613084B2

专利类型

公开/公告日2020-04-07

原文格式PDF

申请/专利权人 MARIANNE MIDDELVEEN;RAPHAEL STRICKER;
展开▼

申请/专利号US201515539442

发明设计人 MARIANNE MIDDELVEEN;RAPHAEL STRICKER;
展开▼

申请日2015-12-23

分类号A61K39/02;A01N63;C12N1/12;G01N33/569;C12Q1/04;C12N1/20;C12Q1/686;

国家 US

入库时间 2022-08-21 11:26:46

相似文献

专利

外文文献

中文文献

1. 西氏鲍与皱纹盘鲍杂交种的DNA分子鉴定方法 [P] . 中国专利： CN101353702B . 2011.01.05

2. 一种西氏鲍与皱纹盘鲍种间杂交制种方法 [P] . 中国专利： CN101167444B . 2010.11.24

3. Method of Culturing Borrelia Spirochetes and of Diagnosing Borrelia Infections, and a Diagnostic Kit for Use in the Methods [P] . 美国专利： US2018267038A1 . 2018-09-20

机译：培养疏螺旋体和诊断疏螺旋体感染的方法，以及用于该方法的诊断试剂盒

4. METHOD OF CULTURING BORRELIA SPIROCHETES AND OF DIAGNOSING BORRELIA INFECTIONS, AND A DIAGNOSTIC KIT FOR USE IN THE METHODS [P] . 世界知识产权组织专利： WO2016105479A1 . 2016-06-30

机译：培养疏螺旋体和诊断疏螺旋体感染的方法，以及用于该方法的诊断工具包

5. Set of starters for detection of Borrelia burgdorferi, method for detection and diagnostics of Borrelia burgdorferi, using the kit of starters and the kit for detection of Borrelia burgdorferi [P] . PL423217A1 . 2019-04-23

机译：一套用于检测伯氏疏螺旋体的启动子，用于检测和诊断伯氏疏螺旋体的方法，使用该试剂盒和一种用于检测伯氏疏螺旋体的试剂盒

1. Advances in Laboratory Diagnostic Methods for Duck Circovirus Infection [J] . Ma Li, Yang Limei, Mei Jianguo, 动物与饲料科学：英文版 . 2015,第003期

2. An In Vitro Blood-Feeding Method Revealed Differential Borrelia turicatae (Spirochaetales: Spirochaetaceae) Gene Expression After Spirochete Acquisition and Colonization in the Soft Tick Ornithodoros turicata (Acari: Argasidae) [J] . Journal of Medical Entomology . 2017,第2期

3. Glass capillary tube feeding: A method for infecting nymphal Ixodes scapularis (Acari : Ixodidae) with the Lyme disease spirochete Borrelia burgdorferi [J] . Broadwater AH, Sonenshine DE, Hynes WL, Journal of Medical Entomology . 2002,第2期

4. Detection of Heterogeneity of Borrelia burgdorferi in Ixodes Ticks by Culture-Dependent and Culture-Independent Methods [J] . Guang Xu, Jaime Wesker, Caitlin White, Journal of Clinical Microbiology . 2013,第2期

5. Quantitation of Three Outer Surface Proteins From The Lyme Disease Spirochete, Borrelia Burgdorferi By Nanoparticle Capture / Multiple Reaction Monitoring-Mass Spectrometry. [C] . Paul Russo, Davide Tamburro, Claudia Fredolini, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

6. Elucidating mechanisms for persistent infection by the relapsing fever spirochete Borrelia hermsii. [D] . James, Allison Eavey. 2016

7. Detection of Heterogeneity of Borrelia burgdorferi in Ixodes Ticks by Culture-Dependent and Culture-Independent Methods [O] . Guang Xu, Jaime Wesker, Caitlin White, 2013

8. Detection of Heterogeneity of Borrelia burgdorferi in Ixodes Ticks by Culture-Dependent and Culture-Independent Methods [O] . Xu, Guang, Wesker, Jaime, White, Caitlin, 2013

1. Liver fibrosis in human immunodeficiency virus/hepatitis C virus coinfection: Diagnostic methods and clinical impact [J] . Caterina Sagnelli ,Salvatore Martini ,Mariantonietta Pisaturo . 世界肝病学杂志：英文版（电子版） . 2015,第24期

2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

3. Mucosa-Associated Lymphoid Tissue (MALT) Lymphoma of the Colon in a Patient with iBorrelia burgdorferi/iInfection (Lyme Arthritis) [J] . Timothy Rinden ,Juan C. Manivel ,Peter A. Valen . 临床医学病理报告(英文) . 2021,第12期

4. Infection Prevalence of iBorrelia burgdorferi/iin Ticks Collected from Songbirds in Far-Western Canada [J] . John D. Scott ,Lance A. Durden ,John F. Anderson . 动物科学期刊（英文） . 2015,第3期

5. 以梅毒螺旋体特异性抗原TP17、TP0453为诊断抗原的间接ELISA方法的建立和优化 [C] . 肖洁 ,郭刚 ,曾明 . 第三次全国免疫诊断暨疫苗学术研讨会 . 2007

6. 鸡鲍氏志贺氏菌双抗夹心ELISA检测方法的建立及应用 [A] . 李炜 . 2010