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METHOD OF PREPARING THREE-DIMENSIONAL CELL SPHEROID INCLUDING ADIPOSE-DERIVED STEM CELLS AND HEPATOCYTES
METHOD OF PREPARING THREE-DIMENSIONAL CELL SPHEROID INCLUDING ADIPOSE-DERIVED STEM CELLS AND HEPATOCYTES
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机译:包括脂肪来源的干细胞和肝细胞的三维细胞球体的制备方法
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摘要
Provided is a method of preparing a three-dimensional cell spheroid, the method including forming the cell spheroid by co-culturing adipose-derived stem cells or mesenchymal stem cells with hepatocytes. According to the cell spheroid prepared by the method, the secretome secreted by the adipose-derived stem cells affects hepatocyte maturation, and therefore, hepatic functions of the finally formed three-dimensional cell spheroid, i.e., organoid, may be enhanced. Further, a composition including a culture medium of the adipose-derived stem cells may prevent or treat liver diseases including hepatitis, hepatotoxicity, cholestasis, fatty liver, etc., and may enhance hepatic functions.
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