首页>
外国专利>
Post-synthetic orthogonal amidation plus metal catalyzed azide-alkyne cycloaddition click chemistry on siRNA
Post-synthetic orthogonal amidation plus metal catalyzed azide-alkyne cycloaddition click chemistry on siRNA
展开▼
机译:合成后的正交酰胺化加上金属催化的叠氮化物-炔烃环加成反应在siRNA上的点击化学
展开▼
页面导航
摘要
著录项
相似文献
摘要
This invention relates to a process for introducing two or more 2′-modifications into an RNA, wherein the RNA has a 2′-O substituent containing an alkyl ester functional group on one or more ribose rings of a strand and a 2′-O substituent containing an alkyne functional groups on one or more ribose rings on the same strand. The process comprises a) adding an amine compound to the RNA to form amidation reactions with the alkyl ester functional groups; b) dissolving the modified RNA from step (a) in a solvent to form a solution; and c) adding an organic azide and a copper or ruthenium catalyst to the solution obtained in step (b) to form 2′-azide-alkyne cycloaddition reaction products with the alkyne functional groups.
展开▼