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THREE-CHANNEL FLUORESCENCE LOCALIZATION SUPER-RESOLUTION BIOLOGICAL MICROSCOPE SYSTEM AND METHOD
THREE-CHANNEL FLUORESCENCE LOCALIZATION SUPER-RESOLUTION BIOLOGICAL MICROSCOPE SYSTEM AND METHOD
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机译:三通道荧光定位超分辨生物显微镜系统及方法
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摘要
A three-channel fluorescence localization super-resolution biological microscope system includes a microscope main body, an illumination module (2), an imaging module (3) and a three-dimensional nanoscale sample locking module (4). The illumination module (2) is used to realize three channels, wide-field and semi-total internal reflection illumination and total internal reflection illumination and is adjustable in an illumination mode. Illumination light generated by the illumination module (2) illuminates a sample through an objective lens. Fluorescent light emitted from the sample is allocated by a light splitter (8) of a fixed ratio after passing through a first multicolor reflector (6) and a front imaging lens (7), one part is allocated into the three-dimensional nanoscale sample locking module (4), and the remaining part is allocated into the imaging module. A three-channel fluorescence localization super-resolution biological microscope method is disclosed.
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