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Development of Herbicide and Sucking Pest Resistant Plant Kalgin-5 by the Over-Expression of Constitutive Promoters Driven Tetra Gene Construct
Development of Herbicide and Sucking Pest Resistant Plant Kalgin-5 by the Over-Expression of Constitutive Promoters Driven Tetra Gene Construct
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机译:过度表达本构启动子驱动Tetra基因构建的除草剂和吸虫抗性植物[Kalgin-5]
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摘要
Recombinant or synthetic polynucleotide sequences comprising three sucking pest resistant genes including an insecticidal Tma12 gene (SEQ ID NO: 18), a PTA gene (SEQ ID NO: 19) and ASAL gene (SEQ ID NO: 20) and a herbicidal Re-PAT gene (SEQ ID NO: 17), transformed in a mono/dicot plant, that are encoded to provide insecticidal and herbicidal toxin proteins in a transgenic plant with constitutively targeted expression, resulting in the decreased resistance development against insecticidal toxins proteins and increased efficacy against the insect mortality, particularly whitefly and jassid. A method or an assay for detecting the presence of transgenic event Kalgin-5 based on the DNA sequence of the recombinant polynucleotide construct inserted into the genome of the transgenic plant and the genomic sequences flanking the insertion site.
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机译:重组或合成的多核苷酸序列,包含三个吮吸害虫抗性基因,包括杀虫性Tma12基因(SEQ ID NO:18),PTA基因(SEQ ID NO:19)和ASAL基因(SEQ ID NO:20)和除草Re-PAT基因(SEQ ID NO:17),已在单/双子植物中转化,编码后可在组成型靶向表达的转基因植物中提供杀虫和除草毒素蛋白,从而降低了对杀虫毒素蛋白的抗性发展并提高了对昆虫的死亡率,尤其是粉虱和j。一种基于插入到转基因植物基因组中的重组多核苷酸构建体的DNA序列和侧接插入位点的基因组序列来检测转基因事件Kalgin-5的存在的方法或测定法。
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