Primers, Methods and Kits for Diagnosing and Predicting Therapy Response of Cancers by Cold-PCR Based Amplification of Mutation-Rich Regions of KRAS, EGFR and P53

摘要

Nucleic acids primers for use in the detection of mutations in KRAS, EGFR and P53 associated with cancer, and in particular provides nucleic acids and methods employing reaction conditions suitable for use in COLD-PCR and high resolution melting HRM analysis of circulating tumour DNA, particularly from lung and colon cancers. The invention further relates to a combination of KRAS and APC mutations in diagnosing cancer.