Methods Using Cumulus Cells for Identification of Viable Oocytes

摘要

The present invention is directed to non-invasive methods for performing selection of ova, otherwise referred to as oocytes, and/or embryos produced from fertilized oocytes. In an embodiment, performing the selection can include comparing the expression level of a gene in cumulus cell masses harvested from at least two oocytes or at least two embryos, the embryos formed by fertilizing the oocyte. In another embodiment, performing the selection can include comparing the expression level of a gene in a cumulus cell mass harvested from an oocyte or an embryo to a population value. By comparing the expression level of a gene (e.g., using either mRNA expression or protein expression) the embodiments and methods disclosed can provide advantages in selecting an egg for fertilization or an embryo for implantation, such as increasing the chances of successful fertilization and/or live birth. Aspects of this disclosure can provide methods for improving the outcome of in vitro fertilization (IVF) protocols through non-invasive genetic testing using a set of markers for determining or predicting the embryo ploidy status and live birth outcome.