SYSTEMS AND METHODS FOR SINGLE-STRAND BREAK SIGNALING AND REPAIR IN A CELL-FREE SYSTEM

摘要

The present application describes structures, systems, and methods for modeling and analysis of single-strand break (SSB) signaling and repair in a cell-free system. Also provided are methods of making the SSB structures and SSB signaling and repair systems. Methods and systems for identifying modulators of DNA damage response (DDR) activity for SSB repair are also described as well as methods of inhibiting SSB repair.