METHOD FOR IMAGING A SAMPLE USING A FLUORESCENCE MICROSCOPE WITH STIMULATED EMISSION DEPLETION

摘要

A method for imaging a sample using a fluorescence microscope with stimulated emission depletion includes controlling the fluorescence microscope and an imaging process of the fluorescence microscope by a microscope controller. An overview image of a target region is generated with a second spatial resolution prior to the imaging process, the second spatial resolution being lower than a first spatial resolution used for scanning sample segments in the imaging process and higher than a third spatial resolution that has been adapted to an extent of an excitation light distribution. The overview image is analyzed to identify image regions without relevant image information. A radiant flux of the depletion light distribution is reduced within a scope of the imaging process when scanning sample segments which are assigned to the image regions without relevant image information.