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GENE DETECTION CHIP, DETECTION METHOD THEREOF, AND MICROFLUIDIC CONTROL CHIP SYSTEM

机译:基因检测芯片,其检测方法和微流控芯片

摘要

Disclosed are a gene detection chip, in which a gene detection channel is formed by an injection port, a microchannel, a reaction cell, and an exit port. The surface of the reaction cell has an aptamer, and the aptamer is modified with a fluorescent label. A detection method and a manufacturing method of the gene detection chip and a microfluidic control chip system are also disclosed. Upon a gene detection being performed, the fluorescent light of the fluorescent label is firstly quenched, and then a sample solution is introduced into the reaction cell through the injection port. If the sample solution has target gene, the target gene will hybridize and combine with the aptamer on the surface of the reaction cell, so as to recover the fluorescent light; if the sample solution does not have the target gene, the fluorescent light stays in the quenched state.
机译:公开了一种基因检测芯片,其中,基因检测通道由注入口,微通道,反应池和出口形成。反应池的表面具有适体,并且该适体被荧光标记修饰。还公开了基因检测芯片和微流控芯片系统的检测方法和制造方法。在进行基因检测时,首先将荧光标记的荧光猝灭,然后通过注射口将样品溶液引入反应池。如果样品溶液中含有靶基因,则该靶基因将与反应池表面的适体杂交并结合,从而回收荧光。如果样品溶液中没有目标基因,则荧光保持淬灭状态。

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