MULTIPLEXED INSTRUMENT-FREE BAR-CHART SPINCHIP INTEGRATED WITH NANOPARTICLE-MEDIATED APTASENSORS FOR VISUAL QUANTITATIVE DETECTION OF MULTIPLE PATHOGENS

摘要

A point-of-care testing (POCT) quantitative pathogen detection device is provided, without the aid of any detectors. In an illustrative embodiment, a POCT pathogen detection device includes an inlet microwell for receiving a substance, the inlet microwell connected to a distribution channel to distribute the substance to an analyzing component. The device also includes an analyzing component. that includes a first pathogen detection component. The first pathogen detection component includes a platinum nanoparticle-labeled magnetic DNA-probe configured to propel a dye through a bar-chart channel when a pathogen in the substance reacts platinum nanoparticle-labeled magnetic DNA-probe. The platinum nanoparticle-labeled magnetic DNA-probe is configured to react with a first pathogen. The device also includes at least one magnet configured to keep unreacted platinum nanoparticle-labeled magnetic DNA-probe in a sample recognition microwell, thereby inhibiting propulsion of the dye into the bar-chart channel when the pathogen is not detected.