NOVEL REAL-TIME MULTIPLEXED, MULTI-COLOR BIOLUMINESCENCE RESONANCE ENERGY TRANSFER ASSAY, APPARATUS, AND USES THEREOF

摘要

The present invention relates to a novel real-time multiplexed, multi-color BRET assay and apparatus for studying protein-protein interactions and determining various biological activities in live cells, wherein said assay is capable of capturing multiple signals simultaneously from a single sample and simultaneously over one or several wells. The real-time multiplexed, multi-color BRET assay is particularly useful for drug-screening, protein interactions, change of conformation of any channel or receptor subunits within live cells in response to potential drug candidates, thereby indicating activation or inhibition of said channel or receptor.