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EPITOPE-SUBSTITUTED VACCINE FOR USE IN IMPROVING SAFETY AND IMMUNOGENICITY AGAINST DENGUE VIRUSES
EPITOPE-SUBSTITUTED VACCINE FOR USE IN IMPROVING SAFETY AND IMMUNOGENICITY AGAINST DENGUE VIRUSES
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机译:表位替代疫苗,用于改善登革热病毒的安全性和免疫性
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摘要
Isolated mutant dengue virus E protein variants are disclosed. The variant comprises an amino acid sequence that is at least 80% identical to SEQ ID NO: 1 and has one or more amino acid residue substitutions at position corresponding to Asn8 (N8), Arg9 (R9), Val12 (V 12) and/or Glu13 (E13). The variant may comprise an amino acid sequence that is at least 90% identical to the SEQ ID NO: 1 and lack an infection-enhancing antibody-binding motif comprising the amino acid sequence of SEQ ID NO: 28 at domain I. An isolated nucleic acid sequence encoding the variant, a plasmid expressing the variant, a plasmid expressing a virus-like particle comprising the variant, a DNA vaccine, and a method of detecting the presence of a dengue virus in a biological sample are also disclosed.
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机译:公开了分离的突变登革热病毒E蛋白变体。该变体包含与SEQ ID NO:1至少80%相同的氨基酸序列,并且在对应于Asn8(N8),Arg9(R9),Val12(V 12)和/的位置具有一个或多个氨基酸残基取代。或Glu13(E13)。该变体可以包含与SEQ ID NO:1至少90%相同的氨基酸序列,并且在结构域I上缺乏包含SEQ ID NO:28的氨基酸序列的增强感染的抗体结合基序。还公开了编码该变体的氨基酸序列,表达该变体的质粒,表达包含该变体的病毒样颗粒的质粒,DNA疫苗以及检测生物学样品中登革热病毒的存在的方法。
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