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PAYLOAD DELIVERY ACROSS CELL MEMBRANES USING CONTINUOUS FLOW FLUIDIC SYSTEM
PAYLOAD DELIVERY ACROSS CELL MEMBRANES USING CONTINUOUS FLOW FLUIDIC SYSTEM
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机译:使用连续流流体系统跨细胞膜进行有效载荷传递
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摘要
Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.
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