IDENTIFICATION OF MICROORGANISMS USING REFLECTION INFRARED SPECTROSCOPY

摘要

The present disclosure presents methods and systems for the spectral identification of microorganisms using reflection infrared spectroscopy. A background spectrum is acquired to measure a water vapor level of an ambient atmosphere in the absence of a sample. The sample containing the microorganism is brought into contact with an infrared reflective substrate, the sample has intact microbial cells. Spectral data is acquired from the sample using reflection infrared spectroscopy no more than a predetermined time after having acquired the background spectrum. The background spectrum and the spectral data are combined thereby producing modified spectral data. The microorganism is characterized using the modified spectral data.