MODULAR CONSTRUCTION OF SYNTHETIC GENE CIRCUITS IN MAMMALIAN CELLS USING MATHEMATICAL MODELING OF CLOSED LOOP TRANSCRIPTIONAL REPRESSORS

摘要

We hereby disclose and claim a mathematically based system for design and implementation synthetic biology genetic circuitry architecture which uses genetic expression circuits which are linked together to achieve a therapeutic or diagnostic effect. The genetic architecture that enables the reversible and tunable desired gene product output is achieved by genetic control elements upstream and downstream of a promoter region. Reversible and tunable control is achieved through the computational design and pairing of transcriptional repression elements with their cognate transcriptional promoters to enable a broad range of therapeutic and diagnostic applications.