obtaining a rough-type salmonella enteritidis and its genetic modifications for use as an avian vaccine

摘要

the present invention relates to a 3934vac salmonella enteritidis strain to which the waal gene has been deleted to obtain a rough phenotype (3934vac dwaal), the procedure for obtaining and used oligos; in order to reduce toxicity and maintain immunogenicity for its application as a vaccine. Another aspect of the present invention concerns a strain of salmonella enteritidis 3934vac dwaal type, that is, of the rough type, which has been modified to express the type i avian adenovirus fiber gene, also the process for obtaining a salmonella enteritidis strain 3034 vac dwaal that expresses an ava-i fiber gene, the invention also includes the development of a new avian vaccine, live, recombinant, effective and safe virus against ava-i virus developed through an insertion process and the integration of ava i fiber genes in the chromosome of an attenuated and non-pathogenic strain of salmonella enteritidis bacteria.