mechanical tissue fragmentation device for preparing an isolated cell composition and method for preparing an isolated cell composition

摘要

the invention relates to a device for the mechanical fragmentation of tissues which is intended for the preparation of the composition of isolated cells, notably adipocytes, from a tissue sample, notably obtained during liposuction, the said preparation comprising at least a step of mechanical fragmentation of the tissue in the device, said device comprising a motorized support (3) and a container (1), the motorized support (3) comprising a motor having a rotor attached to a coupling member , the container (1) internally comprising a rotating axis (9) that can be defined in axial rotation and comprising radial arms (10, 12) capable of cleaning the inside of the container (1) as the rotating axis ( 9) rotates, the motorized support (3) and the container (1) comprising complementary gripping means (11) that allow the container (1) to be immobilized on the motorized support (3), the member being coupling rod and the rotary axis (9) are configured to allow axial rotation of the rotary axis (9) when the motor rotor is rotating. according to the invention, the radial arms (10, 12) are of two types: rigid reed arms (10) and flexible filament arms (12), notably produced from nylon or metal. a method completes the invention.