target sequence specific change technology using nucleotide target recognition

摘要

the present invention relates to a method for targeting a target nucleotide sequence. the method includes introducing into a cell: (i) related proteins type i-d of crispr cas5d, cas6d and cas7d, or nucleic acids encoding these proteins; and (ii) a guide rNA that includes a sequence complementary to said target nucleotide sequence and a common repetitive sequence derived from the locus of the crispr gene before and after said complementary sequence, or a dna encoding said guide rNA.