method of producing a polypeptide in a cell culture; polypeptide produced according to the method; pharmaceutical composition comprising the polypeptide; cell culture medium; cell culture medium for use in the production of a polypeptide of interest; method for determining an optimized concentration of an amino acid used in a cell culture medium for the production of a polypeptide of interest in a cell culture

摘要

method of producing a polypeptide in a cell culture; polypeptide produced according to the method; pharmaceutical composition comprising the polypeptide; cell culture medium; cell culture medium for use in the production of a polypeptide of interest; method for determining an optimized concentration of an amino acid used in a cell culture medium for the production of a polypeptide of interest in a cell culture this invention relates to methods for rationally designing cell culture media for use in cell cultures, for example example, cell cultures used in the production of polypeptides; cell culture media designed with the methods presented; methods of producing a polypeptide of interest, for example, an antibody, using these means; polypeptides produced using the methods and means presented here; and pharmaceutical compositions containing these polypeptides. the rationally designed media contains a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest. rationally designed media can contain a concentration adjusted to the baseline, a, of at least one amino acid which is calculated according to the formula a = [(mx) + (n, p) + (ymx)] .f, where x is the concentration of the amino acid that is used per unit of cell mass, p is the concentration of the amino acid that is used for incorporation into the polypeptide of interest per unit of polypeptide title, m is the multiplier for the desired peak cell density of the culture cellular, n is the multiplier for the desired concentration of the polypeptide of interest, y is the cell maintenance factor; and f is a baseline factor. these rationally designed media can also be used to produce a starting cell culture medium comprising a concentration, b, of at least one amino acid according to the formula b = [a- (zv)] / (1-v), in that z is a concentration of the amino acid in the feed cell culture medium, and v is a volume of the feed culture medium as a proportion of the desired cell culture medium volume.