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Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos
Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos
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机译:生产不育鱼类和产卵水生动物并将化合物送入卵和胚胎的方法
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摘要
Methods for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of compounds that lead to the failure of fertile gonadal development. Compounds may be delivered to the eggs prior to fertilization or water activation or post fertilization and water activation by contacting unfertilized or pre-water-activated fertilized eggs or fertilized eggs in an immersion medium including the compound of interest. Compounds may be conjugated with a molecular transporter compound effective for chorionic transport of the conjugate. The compounds may be antisense Morpholino oligomers that are capable of effectively suppressing the expression of the dead end gene or other essential genes for germ cell development in fish and other egg-producing aquatic animals.
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