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Methods for preventing disulfide bond reduction in cell culture harvest with selenite
Methods for preventing disulfide bond reduction in cell culture harvest with selenite
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机译:防止亚硒酸盐细胞培养收获物中二硫键还原的方法
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摘要
This disclosure relates to methods for the prevention of the reduction of disulfide bonds in a polypeptide expressed in a recombinant host cell, comprising, following fermentation, adding selenite and/or its salts or derivatives to a harvest solution of the recombinant host cell, wherein the disulfide bond in the polypeptide remains non-reduced.
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