DETERMINATION OF A GENDER OF LARVA OF PARASITE TRICHINELLA SPP. BY A METHOD OF LUMINISCENCE

摘要

The invention relates to the field of biology and may be applied for the determination of the sex of the parasite Trichinella spp. larval. The proposed method comprises: (a) providing a sample of Trichinella spp. nematode; (b) fixation of the resultant sample for one hour using Bouin's fixation solution followed by rinsing and storing in tubes in 70% alcohol at a temperature of 0 °C to + 4 °C; (c) prior to staining, incubation of the sample of Trichinella spp. at room temperature for 15 min; (d) staining the sample with 3-N- (2-piperidylacetamido) -benzantrone (AZPP); (e) visualization of the prepared sample by confocal laser scanning microscopy using laser radiation with a wavelength ? = 488 nm with a FITC filter (fluorescence ? = 500-550 nm) and a laser radiation with a wavelength ? = 561 nm with a TRITC filter (fluorescence ? = 570-620 nm); (i) measuring the length of the gut end in the sample; (f) determination of the sex of the Trichinella spp. nematode by the gut end length variation.