Method for diagnosing acute poisonings of chemical etiology

摘要

The invention relates to medicine, in particular to toxicology and can be used for diagnosing acute poisonings of chemical etiology.Summary of the invention consists in that a blood sample of patients with acute poisoning of chemical etiology is treated with Triton X-100, stirred by shaking, for 60 s, is added a solution of phosphate-citrate buffer with a pH of 7.0…7.1, is centrifuged; is transferred 300 µl of hemolysate to a photometric microplate, is measured the Abs1 absorption at a wavelength of 630 nm, is added acetone-cyanohydrin (final concentration 7.5…5.0 µM/L), is stirred for 3 min in a shaker-thermostat and Abs2 absorption is measured at a wavelength of 630 nm, Abs3 absorption at a wavelength of 620 nm, Abs4 absorption at a wavelength of 650 nm. Potassium ferricyanide is added (final concentration 1.1…2.2 µM/L), is stirred for 3 min in a shaker-thermostat and Abs5 absorbance is measured at a wavelength of 540 nm, after which is determined the concentration of MetHb, Hbtot(without SHb), HbO2, SHb, Hbtot in the hemolysate, %MetHb/Hbtot, %SHb/Hbtot, and the sum of inactive blood pigments. In the case when the percentage of methemoglobin in relation to the total amount of Hb is more than 1%, the percentage of sulfohemoglobin in relation to the total amount of Hb is more than 0.4%, and the sum of inactive blood pigments is more than 20%, the presence of acute poisoning of chemical etiology is diagnosed.