Method to obtain peptide part of smooth emission mucin with antihypertensive ability: verify in vivo activity.

摘要

The invention is a part of the value-added part of smooth emitting visceral protein, and aims at preparing hydrolysate and concentrating peptide part of antihypertensive peptide. Three kinds of protein hydrolysates of smooth emitter, hpv-n, hpv-e and hpv-p were prepared by the action of neutral enzyme.Espesse and purafectpart Anti ECA activity was detected in the three hydrolysates, and the highest activity was obtained by purafect  action. The three hydrolysates were separated by ultrafiltration, and the cut-off threshold of the membrane ranged from 50-1k. Five different molar mass fractions (mm) are obtained, which are called fi 50 kDa,FII=50-5 kdaEscape ("5-3 kDa")FIV = 3-1 kDa and FV = 1 kDa. Strong anti ECA activity was observed in all fractions of low molecular weight peptides, especially FIV and FV. The invention also relates to the identification of potential active peptides in the peptide portion. For example, there are six components from UF membrane, which have strong activity (i.e.fiv and FV of each hydrolysate),It was further fractionated by high performance liquid chromatography on a reversed phase C18 silica column (RP-HPLC). By analyzing the very active subfractions, new peptide sequences can be identified, many of which have the homogeneity of antihypertensive peptides described in the literature. The invention also relates to in vivo identification.Inhibitory effect of peptides on partial hypertension After the peptide was injected into hypertensive rats, the whole body blood pressure decreased, showing the power of anti hypertension. The results of the present invention demonstrate the benefits of producing a protein concentrate from the viscera of an emitted, antihypertensive biopeptide. It can be used as food additive and / or in functional food development to prevent hypertension. EM gt keywords lt/Keywords protein hydrolysate; peptide part;Keywords ultrafiltration method; anti ECA peptide; angiotensin-1 converting enzyme; antihypertensive activity;Research on structure and function