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Eukaryotic expression vectors that include regulatory elements of globin gene clusters
Eukaryotic expression vectors that include regulatory elements of globin gene clusters
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机译:包含珠蛋白基因簇调控元件的真核表达载体
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摘要
An in vitro method for recombinantly producing a polypeptide of interest, comprising the steps of (a) providing a host cell comprising an expression cassette comprising, functionally linked together, (i) a locus control region comprising the minus the core element of DNase I hypersensitivity site 2 (HS2) of the human β-globin gene pool; (ii) a promoter region comprising at least a functional part of the promoter of the human Aγ globin gene; and (iii) a coding region comprising a nucleic acid sequence that encodes the polypeptide of interest; (b) culturing the host cell under conditions in which the host cell expresses the polypeptide of interest; and (c) isolating the polypeptide of interest.
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