METHOD FOR CONSTRUCTING VARIABLE REGION SEQUENCE LIBRARY, SEQUENCING METHOD, AND KIT THEREOF

摘要

Provided is a method for constructing a variable region sequence library, comprising: (1) obtaining a DNA sample comprising an encoding variable region; (2) using the DNA sample as a template, and amplifying and encoding a DNA sequence of the variable region by using a first primer group to obtain a first amplification product library; and (3) using the first amplification product library as a template, and amplifying and encoding the DNA sequence of the variable region by using a second primer group and a primer of a first joint, thus obtaining a variable region sequence combination product. Further provided are a kit for constructing the variable region sequence library, and a sequencing method for a variable region sequence.