STEM CELL LINES CONTAINING ENDOGENOUS, DIFFERENTIALLY-EXPRESSED TAGGED PROTEINS, METHODS OF PRODUCTION, AND USE THEREOF

摘要

The present invention provides stably tagged cells, including stem cells and methods for producing such cells comprising one or more tagged, differentially-expressed proteins using a gene editing system. The methods described herein enable the insertion of large fluorescent tags into a plurality of genomic loci to generate stem cells that are phenotypically and functional similar to the un-modified parent population. Stem cells produced by the methods described herein additionally retain the capacity to self-renew and differentiate into specialized cell types and can be used in assays and visualization of three-dimensional live cell imaging.