METHOD FOR SPECIFICALLY KNOCKING OUT HUMAN MER6 GENE BY MEANS OF CRISPR-CAS9, AND SPECIFIC SGRNA THEREOF

摘要

Provided are a method for specifically knocking out a human MER6 gene by means of CRISPR-Cas9, and a specific sgRNA thereof. By means of computer simulation, computation, and design, a group of sgRNAs specifically knocking out a specifically targeted MER6 gene in the human MER6 gene by using the CRISPR-Cas9 is synthesized, the sgRNA and a linear PX330 plasmid are separately connected to be a vector, the cell is transfected, i.e., the knockout of the MER6 gene can be realized. The sgRNA has the advantage that mass production can be performed with a small amount of synthesized polynucleotide fragments; a preparation method is simple and easy to perform; the sgRNA has good targetability; and the knockout efficiency of a CRISPR-Cas9 system is high.