METHOD FOR CONSTRUCTING CHO CELL STRAIN WITH SITE-DIRECTED INSERTION OF CT1.1 GENE AND USE THEREOF

摘要

Disclosed are a method for constructing a CHO cell strain with site-directed insertion of the CT1.1 gene and a use thereof. The cell strain is obtained by constructing an sgRNA expression vector based on CRISPR/Cas9 system according to the nucleotide sequence of an insertion site; constructing, according to an sgRNA action site, an expression cassette that contains the CT1.1 gene and can be integrated into a host genome; then co-transfecting the constructed sgRNA expression vector and a linearized expression cassette into CHO cells; and screening to obtain the CHO cell strain with site-directed insertion of the CT1.1 gene. The cell strain can maintain the efficient and stable expression of the CT1.1 protein for a long period of time.