SYSTEMS AND METHODS TO PERFUSE ISOLATED TISSUE

摘要

Systems and methods to perfuse isolated tissue are provided to avoid ischemia-related tissue damage over an extended perfusion time is provided. A system can comprise a reservoir sized and dimensioned to contain a perfusate. A perfusion supply line can comprise a pump, a cooling system, an oxygenator, and at least one filter. The perfusion supply line can be in fluid communication with the reservoir to draw the perfusate from the reservoir and cool and oxygenate the perfusate. The system can also include an arterial line in fluid communication with the perfusion supply line to direct perfusate from the reservoir to the isolated tissue. A venous outflow can be in fluid communication with the isolated tissue to remove the perfusate from the isolated tissue. A perfusion return line can be in fluid communication with the venous outflow to return the perfusate from the isolated tissue to the reservoir.