The present invention relates to an integrated opto-microfluidic device for real-time detection of gases in biosamples and liquids. The device comprises two different modules, a particle separation module and an optical detection module, where the particle free sample (blood) is separated from particles (blood cells) present in the original sample (blood sample)and infused into the detection module. The particle-free sample reacts with a sensitive chemical probe to provide a fluorescence (or other optical) signal upon excitation using a light source such as laser, and the signal is detected using suitable optoelectronics.
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