METHOD FOR CONSTRUCTING ANTIGEN-PRESENTING CELL LINE WITHOUT ENDOGENOUS HLA GENE BACKGROUND, ANTIGEN-PRESENTING CELL LINE AND USE THEREOF

摘要

Provided is a method for constructing an antigen-presenting cell line without an endogenous HLA gene background, comprising: using a CRISPR/Cas9 gene editing system to knock out an endogenous HLA-C gene from a C1R antigen-presenting cell of a single HLA type, wherein the C1R antigen-presenting cells comprise genes HLA-A, HLA-B and HLA-C, the HLA-A not expressing, and the HLA-B basically not expressing. Also provided is an antigen-presenting cell line without an endogenous HLA gene background that is obtained by the described method.