METHOD FOR PREPARING CHITOOLIGOSACCHARIDE USING SNAILASE AND APPLICATION THEREOF

摘要

The present invention belongs to the technical field of oligosaccharide production, and particularly relates to a method for preparing a chitooligosaccharide using snailase and application thereof. The method for preparing a chitooligosaccharide using snailase involves increasing enzymatic efficiency by exploring and optimizing specific process conditions for enzymolysis of chitosan by snailase, such as enzymolysis time, enzymolysis temperature, substrate concentration, mass ratio of enzyme to substrate, etc., and involves maintaining properties of the chitooligosaccharide by means of freeze drying, so as to facilitate product preservation and further product use. The method provides a basis for mass production of a chitooligosaccharide using snailase. The chitooligosaccharide prepared has a good ameliorating effect on idiopathic pulmonary fibrosis, as indicated by significantly improved fibroblast activation in a rat model of idiopathic pulmonary fibrosis, and thus will be conducive to the rehabilitation of a patient with idiopathic pulmonary fibrosis.