Construction of microRNA gene-mediated novel tissue engineered nerve and applications thereof in repairing nerve defect

摘要

Applications of a microRNA gene based on one or more microRNA genes of miRNA, miR-21, or miR-222 of the let-7 family in tissue engineered nerve construction and in repairing a peripheral nerve defect. The outer and/or inner surfaces or the interior of pores of a tissue engineered nerve transplant can be covered or adsorbed with a macromolecular material nanosphere carrying an miRNA inhibitor, miR-21, or miR-222 of the let-7 family or a mimic thereof, the macromolecular material consisting of biocompatible fibronectin and heparin. By regulating the expression of the microRNA genes to promote peripheral nerve regeneration and tissue engineered nerve construction, in vitro primary cultured Schwann cell proliferation is effectively promoted and an anti-apoptotic effect is provided with respect to neurons; in vitro experiments show that the grafting of the tissue engineered nerve transplant favors peripheral nerve regeneration and is applicable in treating peripheral nerve damages.