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DUAL VECTOR SYSTEM FOR IMPROVED PRODUCTION OF PROTEINS IN ANIMAL CELLS
DUAL VECTOR SYSTEM FOR IMPROVED PRODUCTION OF PROTEINS IN ANIMAL CELLS
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机译:用于改善动物细胞蛋白质生产的双载体系统
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摘要
The present invention refers to a dual vector system for production of one or more recombinant proteins in cells of insect origin comprising a first viral vector comprising a T7 promoter operably linked to a targeting sequence comprising a non-coding sequence and which expression can suppress one or more proteins essential for virus production, followed by a T7 termination sequence, and a second viral vector comprising a promoter operably linked to a T7 RNA polymerase encoding sequence, and at least one gene sequence located on the first and/or second vector encoding one or more recombinant proteins of interest. The invention further refers to recombinant insect cells comprising the dual vector system and methods for producing recombinant proteins using said dual vector system.
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