CULTURE MEDIUM FOR IMPROVING INDUCTION RATE OF TISSUE CULTURED PLANT, PREPARATION METHOD, AND APPLICATION

摘要

Disclosed in the present invention are a culture medium for improving the induction rate of a tissue cultured plant, a preparation method, and an application. The culture medium comprises: 68-94 parts of WPM medium, 0.05-0.5 parts of zeatin, 0.1-1.5 parts of indole-3-acetic acid, 0.3-1.8 parts of brassinolide, 23-56 parts of sucrose, 2-6 parts of flavonoid, 6-18 parts of calcium phosphate, and 32-68 parts of sodium dodecylbenzenesulfonate. The preparation method comprises: mixing and dissolving the raw materials according to the amounts, adjusting the pH value to 5.4-5.6, and performing sterilization for 15-30 min. The culture medium is used for primary culture of Plumbago auriculata. The culture medium provided by the present invention can effectively improve the induction rate of plants to reach about 95%, and can improve the vigorous degree of subsequently cultured plantlets and improve the survival rate and adaptability thereof; moreover, the preparation method is simple, environment-friendly, and safe; in addition, the culture medium is applied to primary culture of Plumbago auriculata, effectively improving the induction rate and enabling Plumbago auriculata to grow vigorously.