Molecular Marker AhyBscc Closely Linked With The Black Seed Coat Of Peanut And Its Application

摘要

#$%^&*AU2020100809A420200702.pdf#####Abstract This invention discloses a molecular marker AhyBscc closely linked with the black seed coat of peanut, and a specific primer pair of the molecular marker AhyBscc, as described in SEQ ID No: 1 and SEQ ID No: 2. It also discloses a method for identifying the seed coat color of peanut by using the molecular marker AhyBscc, which comprises the following steps: Step 1: Extract DNA from peanut seed or blade; Step 2: Perform PCR amplification for extracted DNA by using specific primer pair; Step 3: Detect the amplified products by non-denaturing polyacrylamide gel electrophoresis. If the characteristic bands with size of 330bp appear, the next generation of seeds of the peanut material to be tested is determined to have black coat. This method is simple and easy to operate, and it can be used in peanut molecular breeding and quality improvement. It can realize the fine locating, separating and cloning of genes, help to improve the efficiency of screening black peanuts, and quickly provide a large number of germplasm resources of new peanut variety.Drawings A B 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 ) 500 bp 250 bp Peanuts with black seed coat Pink seed coat Figure 3 Figure 4 3/4