Colorimetric strip, colorimetric card and test method for quickly testing histamine

摘要

#$%^&*AU2020101875A420200924.pdf#####ABSTRACT The invention belongs to the field of chemical testing, and relates to a colorimetric strip, colorimetric card and test method for quickly testing histamine. The colorimetric strip is prepared by a method including the following steps: (1) preparing a PDA vesicle: mixing and stirring 10,12-pentacosadiynoic acid and 1,2-dimyristoyl-sn-glycero-3-phosphocholine with an organic solvent, then evaporating the organic solvent, adding ultrapure water to dissolve a resulting mixture, then performing ultrasonic dispersion, and subsequently, storing a dispersion liquid at a low temperature in the dark; and then performing ultraviolet irradiation on the dispersion liquid for polymerization to obtain a PDA vesicle dispersion liquid; and (2) assembling a testing colorimetric strip: fixing an adsorption carrier to a substrate, and dropping the PDA vesicle dispersion liquid containing 0.2%-0.8% of trehalose as a chromogenic indication liquid onto the adsorption carrier, to obtain the colorimetric strip. Compared with other testing methods, the colorimetric strip according to the invention is free of complex biochemical modification and instrumental testing, easy to store and carry and low in cost, with visible and simple test results.3/3 250 225- Y=14.2831og 2X+3.011 200- R2 =0.9917 175150125100 1 1 1 1024 4096 16384 65536 Histamine Concentration (pM) FIG. 3