RATIOMETRIC FLUORESCENT PROBE FOR PH MEASURMENT AND PREPARATION METHOD AND USE THEREOF

摘要

#$%^&*AU2020102202A420201022.pdf#####ABSTRACT The present invention discloses a ratiometric fluorescent probe for pH measurement and preparation method and use thereof. The ratiometric fluorescent probe has a structural formula of OOH 00 CS-NA . The preparation method for the fluorescent probe 5 of the present invention is simple and has a high productivity, thus it is suitable for promotion and application at a large scale. The fluorescent probe has very weak fluorescence under neutral conditions and a solution thereof in water or organic solvent is light yellow in color. As the solution is increasingly acidic, the color fades gradually and turns into blue-green, and the solution shows increasing fluorescence. The fluorescent probe of the present invention can measure a pH in a range 10 of pH 2-7 and can result in a desired linear relationship at pH 2-4. Measurement with the probe can eliminate background interference effectively with increased sensitivity and accuracy.1/2 j 415 2246 20 S72788 814 *7M 58 10714694 =!091 15260126 1 .7312 FIG. 1 O.3pH=2 0.2cu C ~0.1 400 So 60 70 800 Wavelength/m FIG. 2